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Part:BBa_K4274006:Design

Designed by: Jiawen Chen   Group: iGEM22_KEYSTONE   (2022-09-30)


PvanP*


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For the constitutive expression of sgRNA (patB gene and sfp gene) in pJOE8999, the semi-synthetic promoter PvanP* was used [1]. Two fragments for the deletion of the rnc gene were inserted, one with fabG and acpA homology and the other with smc gene.


Source

Corynebacterium glutamicum

References